Prime Pro 48 Real-time qPCR machine
Prime Pro 48 Real-time qPCR machine
The Prime Pro 48 real time qPCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.
The capacity of Prime Pro 48 accommodates a unique and economical 48-well qPCR plate. The well format mirrors a 384-well plate therefore allowing the use of 16-channel pipettes. The small qPCR plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity and greatly reduces run times.
Key benefits
Speed
• 40 cycles in 40 minutes before optimisation.
• 40 cycles in 15 minutes when optimised.
• Genotype in 4 minutes with over 99% accuracy.
• Set up a 4plex reaction in only 4 mouse clicks.
Technical Specification
| High Resolution Melt | Yes |
|---|---|
| Volume per well | Validated for 5 to 20 ul |
| Detection sensitivity | 1 copy |
| Temp uniformity | +- 0.1°C |
| Temp accuracy | +- 0.15°C @ 60°C and 95°C |
| Temperature range | 30 to 100 degC |
| Average ramp rate | 5.5 degC sec |
| Thermal system | Hollow silver block peltier-based with conductive fluid |
| Block format | 48-well block |
| Consumables | 48-well custom plates and optical adhesive seals |
| Optical system | Dual LED excitation (452 to 486 nm and 542 to 582 nm) |
| Camera | CCD camera |
| Filters | 4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm) |
| Calibrated dyes | SYBR FAM HEX ROX Cy5 |
| Additional dyes | All dyes within range compatible with no additional calibration |
| Passive reference dyes | Use of ROX is supported but optional |
| Data collection | Data always collected in all four filters for all wells |
| Plate setup | Plate setup for data analysis can be altered after run completes |
| Melt curve | Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times |
| PCR cycle time (unoptimised) | 40 cycles in less than 40 minutes |
| PCR cycle time (FAST optimised) | 40 cycles in less than 20 minutes |
| Dynamic range | Greater than 9 logs |
| Calibration | Not required |
| Installation | Plug and play design |
| Precision | Discriminates 5000 and 10000 template copies with 99 percent confidence |
| Warranty | 1 year (parts and labour included) |
| Voltage | 100 to 240 V.AC |
| Frequency | 50 and 60 Hz |
| Nominal current draw | 5A |
| Peak power | 500W (typical power is 180W) |
| Software | Multiple license software included at no additional cost |
| Chemistry | All chemistries supported |
| Applications | Absolute Quantification and Relative Quantification |
| Applications | Allelic Discrimination and High Resolution Melt |
| Dimensions closed (WxDxH) | 34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches) |
| Dimensions open (WxDxH) | 34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches) |
| Weight | 13.6 Kg (30 lb) |
