Chromogenic Salmonella Agar medium is used for the detection and identification of Salmonella species. After pre-enrichment with Buffered Peptone Water 90ml (BPW) for 24 hours in an oven at 37 C, the agar is inoculated and kept in an incubator at 35+-2 C for 18-24 hours.

All the magenta-purple colonies formed were Salmonella spp. indicates its presence.

XLD AGAR

XLD agar was developed for the isolation and differentiation of gram negative enteric bacteria, especially Salmonella spp and Shigella spp.

It is generally used in the isolation and verification stages of Salmonella species. Sowing is done by smear from direct dilution or by drawing from dilution or from a sample taken from the first seeded agar.

Incubation time and temperature is 18-48 hours at 30-35C.

Red and transparent colonies with black centers are typical for Salmonella.

Because the colonies are transparent, blackening in the center can cause the entire colony to appear black.

XLT4 Agar

XLT4 Agar is used as a selective solid medium for analysis of pathogenic Enterobacteriaoeae and especially Salmonella.

Sowing is done by spreading method. After 18—24 hours incubation at 35-37°C

Salmonella colonies appear black due to hydrogen sulfide formation.