DETECTION OF LlSTERIA MONOCYTOGENES

Listeria spp. and L. Monocytogenes as a modern and rapid method in analysis

Chromogenic Listeria Agar can be used, while Oxford Agar can be used as the classical method.

The enrichment fluids for both media are the same.

CHROMOGENIC LISTERIA AGAR BASE

Typical colonies of L. monocytogenes are bluish green on the medium, clear around

It is seen as colonies with colored zones, whereas other Listeria species

There is no zone around the colonies it forms. Incubation temperature and time

It is 24-48 hours at 35-37 C.

OXFORD AGAR

35 +-2 C for 24 — Black colonies, and colony after 48 hours of incubation

blackening, which can also be seen in the surrounding medium, is typical of L. monocytogenes colon-

specifies what.